Walter Scott, Jr. College of Engineering

Graduate Exam Abstract

Ishan Thakkar
M.S. Final
Mar 26, 2013, 3:15 pm
Military Science 200
A plastic microfluidic device for enzymatic biosensors
Abstract: A novel microfluidic device is fabricated for
application to field-portable, low scatter and
economical chemical sensors and biosensors with
excellent detection limits. To evaluate its
utility as a chemical sensor, two pH-sensors based
on two different methods of depositing
fluorescenamine are demonstrated. As a proof-of-
concept of the device’s application as a
biosensor, an enzymatic Hydrogen Peroxide
(H2O2)sensor is demonstrated using O2-sensitive
Ru(dpp) dye as a transducer and catalase enzyme as
a bio-analytical component. The H2O2 sensor
exhibits a phosphorescence to scattered excitation
light ratio of 100. Also, the H2O2 sensor has a
detection limit of 1.2 µM, which is comparable to
the best reported results of H2O2 sensors in
Adviser: Dr Kevin Lear
Co-Adviser: N/A
Non-ECE Member: Dr Kenneth Reardon, Department of Chemical and Biological Engineering
Member 3: Dr George Collins, ECE
Addional Members: N/A
Program of Study:
ECE 441
ECE 471
ECE 536
ECE 548
ECE 569
ECE 573
ECE 699
STAT 511