Ishan Thakkar
M.S. FinalMar 26, 2013, 3:15 pm
Military Science 200
A plastic microfluidic device for enzymatic biosensors
Abstract: A novel microfluidic device is fabricated for
application to field-portable, low scatter and
economical chemical sensors and biosensors with
excellent detection limits. To evaluate its
utility as a chemical sensor, two pH-sensors based
on two different methods of depositing
fluorescenamine are demonstrated. As a proof-of-
concept of the device’s application as a
biosensor, an enzymatic Hydrogen Peroxide
(H2O2)sensor is demonstrated using O2-sensitive
Ru(dpp) dye as a transducer and catalase enzyme as
a bio-analytical component. The H2O2 sensor
exhibits a phosphorescence to scattered excitation
light ratio of 100. Also, the H2O2 sensor has a
detection limit of 1.2 µM, which is comparable to
the best reported results of H2O2 sensors in
literature.
application to field-portable, low scatter and
economical chemical sensors and biosensors with
excellent detection limits. To evaluate its
utility as a chemical sensor, two pH-sensors based
on two different methods of depositing
fluorescenamine are demonstrated. As a proof-of-
concept of the device’s application as a
biosensor, an enzymatic Hydrogen Peroxide
(H2O2)sensor is demonstrated using O2-sensitive
Ru(dpp) dye as a transducer and catalase enzyme as
a bio-analytical component. The H2O2 sensor
exhibits a phosphorescence to scattered excitation
light ratio of 100. Also, the H2O2 sensor has a
detection limit of 1.2 µM, which is comparable to
the best reported results of H2O2 sensors in
literature.
Adviser: Dr Kevin Lear
Co-Adviser: N/A
Non-ECE Member: Dr Kenneth Reardon, Department of Chemical and Biological Engineering
Member 3: Dr George Collins, ECE
Addional Members: N/A
Co-Adviser: N/A
Non-ECE Member: Dr Kenneth Reardon, Department of Chemical and Biological Engineering
Member 3: Dr George Collins, ECE
Addional Members: N/A
Publications:
N/A
N/A
Program of Study:
ECE 441
ECE 471
ECE 536
ECE 548
ECE 569
ECE 573
ECE 699
STAT 511
ECE 441
ECE 471
ECE 536
ECE 548
ECE 569
ECE 573
ECE 699
STAT 511