Ming-Hao Cheng
Ph.D. FinalOct 18, 2024, 11:00 am - 1:00 pm
LSC 394
IMSIS: An Instrumented Microphysiological System with Integrated Sensors for Monitoring Cellular Metabolic Activities
Abstract: Well plates are widely used in biological experiments, particularly in pharmaceutical sciences and cell biology. Its popularity stems from its versatility to support a variety of fluorescent markers for high throughput monitoring of cellular activities. However, using fluorescent markers in traditional well plates has its own challenges, namely, they can be potentially toxic to cells, and thus, may perturb their biological functions; and it is difficult to monitor multiple analytes concurrently and in real-time inside each well. This dissertation presents a fully instrumented microphysiological system with integrated sensors (IMSIS) with a similar well format. Each well in the microphysiological system has a set of sensors for monitoring multiple metabolic analytes in real-time. The IMSIS platform is supported by integrated bioelectronic circuits and a graphical user interface for easy user configuration and monitoring. The system has integrated microfluidics to maintain its microphysiological environment within each well. The IMSIS platform currently incorporates O2, H2O2, and pH sensors inside each well, allowing up to six wells to perform concurrent measurements in real-time. Furthermore, the architecture is scalable to achieve an even higher level of throughput. The miniaturized design ensures portability, suitable for small offices and field applications.
The IMSIS platform was successfully used to monitor in real-time the mitochondrial functions of live bovine embryos in O2 consumption, H2O2 release as an indication of ROS production, and extracellular acidity changes before and after the introduction of external substrates.
The IMSIS platform was successfully used to monitor in real-time the mitochondrial functions of live bovine embryos in O2 consumption, H2O2 release as an indication of ROS production, and extracellular acidity changes before and after the introduction of external substrates.
Adviser: Thomas Chen
Co-Adviser: NA
Non-ECE Member: Elaine Carnevale, Biomedical Sciences
Member 3: Adam Chicco, Biomedical Sciences
Addional Members: Kevin Lear, Jesse Wilson
Co-Adviser: NA
Non-ECE Member: Elaine Carnevale, Biomedical Sciences
Member 3: Adam Chicco, Biomedical Sciences
Addional Members: Kevin Lear, Jesse Wilson
Publications:
[J1.] (Under review) Fresa, K., Cheng, M.-H., Crook, A., Saviola, A., Chen, T., Carnevale, E. (2024) Delayed Blastocyst Development is Associated with Altered Metabolism and Proteome in Male and Female Bovine Embryos. Biology of Reproduction.
[J2.] (In progress) Cheng, M.-H., Fresa, K., Gonzalez-Castro, R., Chicco, A., Carnevale, E., Chen, T. (2024). Assessing metabolism of bovine embryos with normal or delayed development. Biosensors and Bioelectronics.
[J3.] (Under review) Fresa, K., Catandi, G., Gonzalez-Castro, R., Omar, A., Whitcomb, L.A., Cheng, M.-H., Chen, T., Carnevale, E.M., Chicco, A.J. (2024). Impact of dietary essential fatty acids on blood, muscle and follicular cell phospholipid composition and mitochondrial function in aged mares. Scientific Reports.
[J4.] (Under review) Way, R., Templeton, H., Ball, D., Cheng, M.-H., Tobet, S.A., Chen, T. (2024). A Microphysiological System for Studying Barrier Health of Live Tissues in Real Time. Nature Communications Engineering.
[J5.] Cheng, M.-H., Way, R., Catandi, G., Fresa, K. Carnevale, E., Chicco, A.J., Chen, T. (2024). IMSIS: An Instrumented Microphysiological System with Integrated Sensors for Monitoring Cellular Metabolic Activities. Biosensors and Bioelectronics.
[J6.] Catandi, G., Fresa, K., Cheng, M.-H., Whitcomb, W., Broeckling, C.D., Chen, T., Chicco, A.J., Carnevale, E. (2024). Follicular metabolic alterations associated with obesity in mares can be mitigated by dietary supplementation. Scientific Reports.
[J7.] Catandi, G., Cheng, M.-H., Chicco, A.J., Chen, T., Carnevale, E. (2023). L-Carnitine Enhances Developmental Potential of Bovine Oocytes Matured Under High Lipid Concentrations In Vitro. Animal Reproduction Science.
[J8.] Cheng, M.-H., Chicco, A.J., Ball, D. & Chen, T.W. (2022). Analysis of Mitochondrial Oxygen Consumption and Hydrogen Peroxide Release from Cardiac Mitochondria Using Electrochemical Multi-Sensors. Sensors and Actuators B: Chemical.
[J9.] Obeidat, Y.M., Cheng, M.-H., Catandi, G., Carnevale, E., Chicco, A.J., & Chen, T. (2019). Design of A Multi-Sensor Platform for Integrating Extracellular Acidification Rate with Multi-Metabolite Flux Measurement for Small Biological Samples. Biosensors and Bioelectronics.
[C1.] (Submitted) Catandi, G., Fresa, K., Gonzalez-Castro, R., Cheng, M.-H., Chen, T., Chicco, A., Carnevale, E., “Effects of Feeding Nonstructural Carbohydrates on the Metabolic Function of Oocytes and Follicle Cells in Older Mares,” 70th American Association of Equine Practitioners Convention, Orlando, Florida, December 7 – 11, 2024.
[C2.] (Accepted) Cheng, M.-H., Zimara, C., Jannaman, E, Schoolcraft, W., Yuan, Y., Chen, T.W., “Non-invasive single blastocyst metabolic assessment revealed quiescent metabolic activity associated with improved developmental potential,” American Society for Reproductive Medicine Scientific Congress & Expo, Denver, Colorado, October 19-23, 2024.
[C3.] Catandi, G., Fresa, K., Cheng, M.-H., Chen, T., Chicco, A., Carnevale, E., “Effects of Feeding Oils and Supportive Nutrients on Skeletal Muscle and Oocyte Metabolism in Old Mares,” 69th American Association of Equine Practitioners Convention, San Diego, California, November 29 – December 3, 2023.
[C4.] Cheng, M.-H., Patlin, B., Tobet, S.A., Chen, T.W., “Real-time Measurement of Metabolism in Precision Cut Lung Slices,” Thomas L. Petty Aspen Lung Conference, Aspen, Colorado, June 6-9, 2023.
[C5.] Catandi, G., Fresa, K., Cheng, M.-H., Chen, T., Chicco, A., Carnevale, E., “Follicular and Systemic Metabolic Alterations in Obese Mares Can Be Mitigated by Dietary Supplementation,” American Association of Equine Practitioners Convention, San Antonio, Texas, November 18-22, 2022.
[C6.] Cheng, M.-H., Jannaman, E., Trowbridge, K., Reed, L., Schoolcraft, W., Chicco, A., Yuan, Y., Carnevale, E., Chen, T.W., “Novel Microsensors Revealed the Impact of High Maternal Body Weight and Advanced Maternal Aging on Individual Human Oocyte Metabolic Function,” American Society for Reproductive Medicine Scientific Congress & Expo, Anaheim, California, October 22-26, 2022.
[C7.] Cheng, M.-H., Chicco, A.J., Patlin, B., Tobet, S.A., Chen, T.W., “A Novel Multi-Sensors Platform for Real-Time Measurement of Cell/ Tissue Metabolism,” The EACR Conference on Cancer Metabolism, Bilbao, Spain, October 11-13, 2022.
[J1.] (Under review) Fresa, K., Cheng, M.-H., Crook, A., Saviola, A., Chen, T., Carnevale, E. (2024) Delayed Blastocyst Development is Associated with Altered Metabolism and Proteome in Male and Female Bovine Embryos. Biology of Reproduction.
[J2.] (In progress) Cheng, M.-H., Fresa, K., Gonzalez-Castro, R., Chicco, A., Carnevale, E., Chen, T. (2024). Assessing metabolism of bovine embryos with normal or delayed development. Biosensors and Bioelectronics.
[J3.] (Under review) Fresa, K., Catandi, G., Gonzalez-Castro, R., Omar, A., Whitcomb, L.A., Cheng, M.-H., Chen, T., Carnevale, E.M., Chicco, A.J. (2024). Impact of dietary essential fatty acids on blood, muscle and follicular cell phospholipid composition and mitochondrial function in aged mares. Scientific Reports.
[J4.] (Under review) Way, R., Templeton, H., Ball, D., Cheng, M.-H., Tobet, S.A., Chen, T. (2024). A Microphysiological System for Studying Barrier Health of Live Tissues in Real Time. Nature Communications Engineering.
[J5.] Cheng, M.-H., Way, R., Catandi, G., Fresa, K. Carnevale, E., Chicco, A.J., Chen, T. (2024). IMSIS: An Instrumented Microphysiological System with Integrated Sensors for Monitoring Cellular Metabolic Activities. Biosensors and Bioelectronics.
[J6.] Catandi, G., Fresa, K., Cheng, M.-H., Whitcomb, W., Broeckling, C.D., Chen, T., Chicco, A.J., Carnevale, E. (2024). Follicular metabolic alterations associated with obesity in mares can be mitigated by dietary supplementation. Scientific Reports.
[J7.] Catandi, G., Cheng, M.-H., Chicco, A.J., Chen, T., Carnevale, E. (2023). L-Carnitine Enhances Developmental Potential of Bovine Oocytes Matured Under High Lipid Concentrations In Vitro. Animal Reproduction Science.
[J8.] Cheng, M.-H., Chicco, A.J., Ball, D. & Chen, T.W. (2022). Analysis of Mitochondrial Oxygen Consumption and Hydrogen Peroxide Release from Cardiac Mitochondria Using Electrochemical Multi-Sensors. Sensors and Actuators B: Chemical.
[J9.] Obeidat, Y.M., Cheng, M.-H., Catandi, G., Carnevale, E., Chicco, A.J., & Chen, T. (2019). Design of A Multi-Sensor Platform for Integrating Extracellular Acidification Rate with Multi-Metabolite Flux Measurement for Small Biological Samples. Biosensors and Bioelectronics.
[C1.] (Submitted) Catandi, G., Fresa, K., Gonzalez-Castro, R., Cheng, M.-H., Chen, T., Chicco, A., Carnevale, E., “Effects of Feeding Nonstructural Carbohydrates on the Metabolic Function of Oocytes and Follicle Cells in Older Mares,” 70th American Association of Equine Practitioners Convention, Orlando, Florida, December 7 – 11, 2024.
[C2.] (Accepted) Cheng, M.-H., Zimara, C., Jannaman, E, Schoolcraft, W., Yuan, Y., Chen, T.W., “Non-invasive single blastocyst metabolic assessment revealed quiescent metabolic activity associated with improved developmental potential,” American Society for Reproductive Medicine Scientific Congress & Expo, Denver, Colorado, October 19-23, 2024.
[C3.] Catandi, G., Fresa, K., Cheng, M.-H., Chen, T., Chicco, A., Carnevale, E., “Effects of Feeding Oils and Supportive Nutrients on Skeletal Muscle and Oocyte Metabolism in Old Mares,” 69th American Association of Equine Practitioners Convention, San Diego, California, November 29 – December 3, 2023.
[C4.] Cheng, M.-H., Patlin, B., Tobet, S.A., Chen, T.W., “Real-time Measurement of Metabolism in Precision Cut Lung Slices,” Thomas L. Petty Aspen Lung Conference, Aspen, Colorado, June 6-9, 2023.
[C5.] Catandi, G., Fresa, K., Cheng, M.-H., Chen, T., Chicco, A., Carnevale, E., “Follicular and Systemic Metabolic Alterations in Obese Mares Can Be Mitigated by Dietary Supplementation,” American Association of Equine Practitioners Convention, San Antonio, Texas, November 18-22, 2022.
[C6.] Cheng, M.-H., Jannaman, E., Trowbridge, K., Reed, L., Schoolcraft, W., Chicco, A., Yuan, Y., Carnevale, E., Chen, T.W., “Novel Microsensors Revealed the Impact of High Maternal Body Weight and Advanced Maternal Aging on Individual Human Oocyte Metabolic Function,” American Society for Reproductive Medicine Scientific Congress & Expo, Anaheim, California, October 22-26, 2022.
[C7.] Cheng, M.-H., Chicco, A.J., Patlin, B., Tobet, S.A., Chen, T.W., “A Novel Multi-Sensors Platform for Real-Time Measurement of Cell/ Tissue Metabolism,” The EACR Conference on Cancer Metabolism, Bilbao, Spain, October 11-13, 2022.
Program of Study:
CM 505
CM 580A1
BIOM 581B5
STAT 511A
ECE 581B1
ECE 581B2
ECE 581B3
ECE 581B4
CM 505
CM 580A1
BIOM 581B5
STAT 511A
ECE 581B1
ECE 581B2
ECE 581B3
ECE 581B4