Abstract: Mitochondrial diseases affect 1 in 4000 individuals in the U.S. among adults and children of all races and genders. Nevertheless, these diseases are hard to diagnose because they affect each person differently. I will present our effort in utilizing transient absorption microscopy (TAM) to develop a label-free diagnosis method.
My presentation will introduce the BGO crystal imaging results and heme electrophoresis gel signal from a TAM with an ultrafast fiber laser. Then I will show our mitochondria label-free image results using a TAM with a solid-state laser. The results demonstrate that not only can we form mitochondria label-free images from live muscle tissue, but we also can differentiate mitochondria redox states. Lastly, I will show our preliminary results on building a tunable pump laser using supercontinuum generation and future improvements on the current system.

Adviser: Jesse Wilson
Co-Adviser: N/A
Non-ECE Member: Stuart Tobet, Department of Biomedical Sciences
Member 3: Randy Bartels, Electrical and Computer Engineering
Addional Members: Diego Krapf, Electrical and Computer Engineering

Publications:
1st author paper:
Biomedical Optics Express Vol. 13, Issue 4, pp. 2103-2116 (2022).
J. of Biomedical Optics, 25(10), 106503 (2020).
J. Phys. Chem. B 2022, 126, 7, 1404–1412
2nd author paper:
Review of Scientific Instruments 92, 023704 (2021),

Program of Study:
ECE-604-001
ECE-581B7-001
ECE-546-001
ECE-503-001
CHEM-571B-001
CHEM-571A-001
ECE-481A3-001
GRAD-550-001