Graduate Exam Abstract

Caleb Begly

M.S. Final
July 30, 2019, 8:05 am - 10:00 am
Scott 231
Study of Real-Time Spatial and Temporal Behavior of Bacterial Biofilms Using 2D Impedance Spectroscopy

Abstract: The study of biofilms and their effect on disease treatment, prevention, and cures has been increasing in importance in recent years. Bacterial biofilms are colony formations developed by bacteria that allow them to anchor onto a surface and survive hostile environments. The formation of harmful bacteria biofilms on some surfaces can be troublesome, particularly in the case of medical implants. The continuing rise of antibiotic resistant bacteria over the past decade had escalated the need to study and understand biofilms. This thesis presents the design of a multi-channel impedance spectroscopy to allow 2D spatial and temporal evaluation of biofilm growth. The custom-designed circuits allow measurement updates once per second on the entire set of impedance sensors. The distance between the neighboring sensors is 220 micrometers, allowing real-time observation of biofilm growth. The initial results show that proposed 2D impedance spectroscopy provides needed accuracy to predict the existence of bacteria biofilm at a given sensor location. The initial results were validated using optical images with fluorescent staining.

Adviser: Tom Chen
Co-Adviser: N/A
Non-ECE Member: Adam Chicco, Department of Biomedical Sciences
Member 3: Jesse Wilson, Department of Electrical and Computer Engineering, and School of Biomedical Engineering
Addional Members: N/A


Program of Study:
ECE 571/575
ECE 512
ECE 562
ECE 537
ECE 581 (B1 & B5)
GRAD 550
GRAD 544