Amy Pruden - Curriculum vitae

 

Department of Civil Engineering

 

phone: (970) 491-8814

Colorado State University

 

fax: (970) 491-8671

Fort Collins, CO 80523

 

email: apruden@engr.colostate.edu


Professional Appointments

2002-present

Assistant Professor, Department of Civil Engineering, Colorado State University

 

 

1998-2002

Graduate Research Assistant, Department of Civil and Environmental Engineering, University of Cincinnati

 

 

1996-1997

Undergraduate Research Assistant, Department of Biological Sciences, University of Cincinnati


Education

Ph.D., Environmental Science, August 2002, Department of Civil and Environmental Engineering, University of Cincinnati, Cincinnati, OH
Thesis: Biodegradation of Methyl tert-Butyl Ether.

B.S., Biology, June 1997, Department of Biological Sciences, University of Cincinnati, Cincinnati, OH
Minor in Business Spanish; G.P.A. (3.98/4.0) Summa Cum Laude
Honors Thesis: Assessment of Predation Cost of Male Decoration and Courtship Displays in Wolf Spiders Using Video Imaging.

Awards and Honors

NSF CAREER Award, 2006.
Faculty Award for Excellence in Research, CSU Department of Civil Engineering, 2006.
Globe Award for Contribution to Internationalization of Colorado State University, 2005.
ASCE Journal of Environmental Engineering Editor's Prize, 2002.
Departmental Nominee for University Dean's Distinguished Ph.D. Dissertation Fellowship, Department of Civil and Environmental Engineering, 2001.
John David Eye Most Outstanding First Year Graduate Student, University of Cincinnati, Department of Civil and Environmental Engineering, 1999.
Most Outstanding Student in Overall Academic Achievement, University of Cincinnati, Department of Biological Sciences, 1997.
Most Outstanding Presentation for Honors Biology Degree, University of Cincinnati, Department of Biological Sciences, 1997.
Completed Honors Scholars Program, Named Distinguished Honors Scholar, University of Cincinnati, Honors Scholars Program, 1997.
C-Ring Award, Most Outstanding Woman of Class of 1997, University of Cincinnati.
Phi Beta Kappa, 1996.
Undergraduate Research Fellowship, National Science Foundation (NSF), 1996.

 

RESEARCH & CREATIVE ACTIVITY SUMMARY

 

Current Research Projects

 

CAREER: Antibiotic Resistance Genes (ARG) as Emerging Pollutants in Our Water: Pathways, Mitigation, and Treatment, National Science Foundation., Feb. 1, 2006- Jan. 31, 2010. $400,000. P.I.

Role of Animal Waste Composting Protocols in Minimizing the Impacts of Agricultural Antibiotics to the Environment, CSU Agricultural Experiment Station, Sept. 1 2004-Aug. 30 2007. $105,000. P.I., Co-P.I.s: Kenneth H. Carlson and Jessica Davis.

M olecular Biology for Environmental Engineers, National Science Foundation: Course Curriculum and Laboratory Improvement (CCLI), Aug. 1, 2005 – July 31, 2007. $40,000. P.I.

Ten Mile Creek Passive Systems for AMD Remediation: Biological Studies of Luttrell, Peerless Jenny King, Leviathan, and Elizabeth, EQM Management, Inc., Golder Associates, & U.S. EPA., June 1, 2005 – Dec. 31, 2006. $80,000. P.I.

Testing of Micro-Sensor Technology for Security Monitoring of Drinking Water Distribution Systems, ST-Infonox, Inc., July 1, 2006- Jan. 31, 2007. $61,000. Co-P.I., P.I.: Kenneth H. Carlson. Environmental Research Collaboration, SunCor Oil Refinery, March 31, 2005 – Dec. 31, 2006. $170,000. Co-P.I., P.I. Thomas Sale.

Minimizing the Environmental Impact of Agricultural Pharmaceutical Use at a Watershed Level, USDA CREES, Sept. 15, 2003-Sept. 14, 2006. $450,000. Co-P.I., P.I.: Kenneth H. Carlson. Guidance for Stormwater-Borne Solids,   Water Environment Research Foundation (WERF), Oct.   15, 2005-Oct. 14 2006.   $75,000. Co-P.I., P.I.: Larry A. Roesner.

Deep Underground Science and Engineering Laboratory (DUSEL) Conceptual Design, NSF, June-2005-Dec. 31, 2006.   $76,800. Collaborator, P.I.: Chang Kee Jung, SUNY Stony Brook.

Chemical Lumping for Mixtures Modeling, US EPA, Oct. 1 2002-Sept. 30 2006. $700,000. Collaborator, P.I.: Kenneth F. Reardon.

 

Past Research Projects

Environmental Biotechnology for Engineers in the 21 st Century, Colorado Institute of Technology, Oct. 26, 2004-May. 25 2006. $100,000. P.I., Co-P.I.s: Kenneth F. Reardon and George J. Collins.

Development and Characterization of Microbial Inocula for High-Performance Passive Treatment of Acid Mine Drainage, US EPA Hazardous Substance Research Center, Nov. 1 2003-Nov. 1 2005. $195,000. Co-P.I., P.I.: Kenneth F. Reardon.

Assessment of Electrokinetic Injection of Amendments for Remediation of Acid Mine Drainage, US EPA Hazardous Substance Research Center, Nov. 1 2003-Nov. 1 2005. $192,000. Co-P.I., P.I.: Kenneth H. Carlson.

Peer-Reviewed Publications Pending

  1. Pei, R., Cha, J-M., Carlson, K.H., and A. Pruden. Biological Treatment of Antibiotic Resistance Genes (ARG) in Dairy Lagoon Water. Environ. Sci. Technol. (in preparation, submission expected September, 2006).
  2. Hong, H., Inman, L.Y., Pruden, A., and K.F. Reardon. Comparison of CE-SSCP and DGGE for Monitoring a Complex Microbial Community Remediating Mine Drainage. J. Microbiol. Meth. (in preparation, submission expected August, 2006).
  3. Storteboom, H., Kim, S.C., Doesken, K., Davis, J., Carlson, K.H., and A. Pruden. Response of Tetracycline and Resistance Genes tet(W) and tet(O) to High Level and Low Level Manure Management. Env. Microbiol. (Submitted August , 2006).
  4. Pruden, A., Messner, N.L., Pereyra, L.P., Hiibel, S., Hanson, R.E., and K.F. Reardon. Role of Inoculum in the Performance of Sulfate-Reducing Columns Remediating Heavy Metals. Water Research (submitted April, 2006).  

Peer-Reviewed Publications

  1. Pruden, A., Pei, R., Storteboom, H., and K.H. Carlson. Antibiotic Resistance Genes (ARG) as Emerging Contaminants: Studies in Northeastern Colorado. Environ. Sci. Technol. (in press for Special Issue on Emerging Contaminants, Sept. 2006).
  2. Pei, R., Kim, S.C., Carlson, K.H., and A. Pruden. (2006). Effect of River Landscape on the Sediment Concentrations of Antibiotics and Corresponding Antibiotic Resistance Genes (ARG). Water Res. 40(12):2427-2435.
  3. Sharma, A., Pruden, A., Stan, O., and G.J. Collins. Bacterial Inactivation Using An RF Powered Atmospheric Pressure Plasma. (2006). IEEE Transactions on Plasma Science Special Issue on Non-thermal Medical/Biological Applications of Ionized Gases and Electromagnetic Fields 34(4):1-7.
  4. Sharma, A., Pruden, A., Yu, Z., and G.J. Collins. (2005). Bacterial Inactivation In Open-Air By The Afterglow Plume Emitted From A Grounded Hollow Slot Electrode. Env. Sci. Technol. 39(1):339-334.
  5. Rahul, R., Stan, O., Rahman, A., Littlefield, E., Hoshimiya, K., Yalin, A.P., Sharma, A., Pruden, A., Moore, C.A., Yu, Z., and G. J. Collins. (2005). Optical and RF Electrical Characteristics of Atmospheric Pressure Open-Air Hollow Slot Microplasmas and Application to Bacterial Inactivation. J. Phys. D: Appl. Phys.38(11):1750-1759.
  6. Pruden, A., Sedran, M.A., Suidan, M.T., and A.D.Venosa. (2005). Anaerobic Biodegradation of Methyl tert-Butyl Ether Under Iron-Reducing Conditions. Water Environ. Res. 77(3):297-304.
  7. Pruden, A., and M.T. Suidan. (2004). Effect of Benzene, Toluene, Ethyl Benzene, and p-Xylene (BTEX) on the Biodegradation of Fuel Oxygenates Methyl tert-Butyl Ether (MTBE) and tert-Butyl Alcohol (TBA) by Pure Bacterial Culture UC1. Biodegradation15(4): 213-227.
  8. Pruden, A., and G.W. Uetz. (2004). Assessment of Potential Predation Costs of Male Decoration and Courtship Display in Wolf Spiders Using Video Digitization and Playback. Insect Behavior. 17(1): 67-80.
  9. Sedran, M.A., Pruden, A., Wilson, G.J., Suidan, M.T., and A.D. Venosa. (2004). Biodegradation of Methyl tert-Butyl Ether and BTEX at Varying Hydraulic Retention Times. Water. Env. Res.76(1): p47-55.
  10. Zhu, X., Suidan, M.T., Pruden, A., Yang, C., Alonso, C., Kim, B.J., and B.R. Kim. (2004). Effect of Henry’s Constant on Biofilter Performance. J. Air and Waste Mgmt. Assoc.54(4):409-419.
  11. Pruden, A., Sedran, M.A., Suidan, M.T., and A.D. Venosa. (2003). Biodegradation of MTBE and BTEX in an Aerobic Fluidized-Bed Reactor. Water Sci. Technol. 47(9):123–128.
  12. Sedran, M.A., Pruden, A., Wilson, G.J., Suidan, M.T., A.D. Venosa. (2002). Effect of BTEX on the Degradation of MTBE and TBA by a Mixed Bacterial Consortium. ASCE J. Environ. Eng.128: 830-835.
  13. Wilson, G.J., Pruden, A., Suidan, M.T., and A.D. Venosa. (2002). Biodegradation Kinetics of MTBE in Laboratory Batch and Continuous Flow Reactors. ASCE J. Environ. Eng. 128: 824-829.
  14. Pruden, A., Suidan, M.T., Morrison, J., and A.D. Venosa. (2001). Denaturing Gradient Gel Electrophoresis Analysis of a Methyl tert-Butyl Ether Degrading Culture Applied in a Membrane Bioreactor. Water Sci. Technol: Water Supply 2:207-212.
  15. Pruden, A., Wilson, G.J., Suidan, M.T., and A.D. Venosa. (2001). Biodegradation of Methyl tert-Butyl Ether Under Various Substrate Conditions. Environ. Sci. Technol. 35(21):4235-4241.
  16. Wilson, G.J., Pruden, A., Suidan, M.T., and A.D. Venosa. (2001). Aerobic Biodegradation of Gasoline Oxygenates MTBE and TBA. Water Sci. Technol. 43(2):277-284.
  17. Chang, J-Y, Stephen, J.R. Pruden, A., Venosa, A.D., Brüggemann, J., Macnaughton, S.J., Kowalchuk, G.A., Haines, J.R., Kline, E., and D.C. White. (2000). Phylogenetic Analysis of Aerobic Freshwater and Marine Enrichment Cultures Efficient in Hydrocarbon Degradation: Effect of Profiling Method. J. Microbiol. Meth.40:19-31.

Theses and Dissertations

  1. Pruden, A. (2002). Biodegradation of Methyl tert-Butyl Ether. Doctoral Dissertation. University of Cincinnati, Cincinnati, OH.
  2. Pruden, A. (1997). Assessment of Predation Cost of Male Decoration and Courtship Displays in Wolf Spiders Using Video Imaging. Honors Thesis. University of Cincinnati, Cincinnati, OH.

Invited Lectures

  1. Pruden, A. Antibiotics and Antibiotic Resistance Genes (ARG) in the Environment. Soil and Water Conservation Society: International Conference on Conservation & Environmental Management, Keystone, CO, July 22-25, 2006.
  2. Pruden, A. Antibiotic Resistance Genes in the Poudre River Watershed. American Society for Microbiology 106 th General Meeting, Orlando, FL, May 21-25, 2006.
  3. Pruden, A. Biogeochemical Treatment of Mining Influenced Water, DOE Idaho National Laboratory, Idaho Springs, ID, May 11, 2006.
  4. Pruden, A., K.F. Reardon, and Alexis Templeton. Genomic and Proteomic Analysis of Deep Underground Microbial Communities. Henderson Deep Underground Science and Engineering Laboratory (DUSEL) Capstone Workshop, May 4-7, 2006, State University of New York, Stonybrook, NY.
  5. Pruden, A. Potential Impacts of Antibiotics in the Environment, Colorado Soil and Water Conservation Society, Denver, CO, November 15, 2005.
  6. Pruden, A. New Methods of Biochemical and Microbiological Design and Assessment of Bioreactors Treating Metal Contaminants, U.S. EPA Workshop on Abandoned Mine Lands: Treatment Technologies, Denver, CO, May 10-12, 2005.
  7. Pruden, A. Antibiotic Resistance Genes as an Emerging Contaminant, Texas Tech University, Lubbock, TX, March 25, 2005.
  8. Pruden, A. Antibiotic Resistance Genes in the Sediments of the Poudre River, Colorado School of Mines, Golden, CO, February 3, 2005.
  9. Pruden, A. Microbial Community Analysis of Sulfate-Reducing Permeable Reactive Zones Remediating Heavy Metals, Presentation at Microbial Supergroup Meeting, University of Colorado, Boulder, April 13, 2004.
  10. Pruden, A. Fuel Oxygenates, Where Have We Come From, Where are We Going? Presentation to Department of Civil, Environmental, and Architectural Engineering, University of Colorado, Boulder, February, 2003.
  11. Pruden, A. Challenges in MTBE Bioremediation, Colorado State University Sigma Xi Chapter, October, 2002.
  12. Pruden, A. Challenges in Ex Situ MTBE Bioremediation. Presented to the Civil Engineering Department, Rice University, Houston, TX, March, 2002.

 

Book Reviews

  1. Pruden, A. (2006). Review of Biotreatment of Industrial Effluents, by M. Doble and A. Kumar. ASCE Journal of Hydraulic Engineering, 132:116.

 

Conference Proceedings and Oral Presentations

  1. Klein, D.A., Flores, R.M, Venot, C., Pereyra, L.P., and A. Pruden. Aquifer vs. Coal-derived Methanogens in Paleocene Fort Union Formation Coals – Molecular Technique-Based Information and its Ecological Implications. Geological Society of America Annual Meeting, Oct. 22-25, 2006, Philadelphia, PA.
  2. Raynal, M., Sale, T., and A. Pruden. Wetland Treatment of MTBE Contaminated Groundwater at a Local Refinery. M. ASCE Environmental and Water Resources Institute (EWRI) World Environmental and Water Resources Conference, May 21-25, 2006, Omaha, NE.
  3. Pereyra, L.P., Pruden, A., Hiibel, S.R., Inman, L.Y., Kashani, N., Reardon, K.F., and D. Reisman. Microbiology of Two Sulfate-Reducing Field Bioreactors Used for the Remediation of Mining Influenced Water. ASCE Environmental and Water Resources Institute (EWRI) World Environmental and Water Resources Conference, May 21-25, 2006, Omaha, NE.
  4. Pei, R., Storteboom, H., and A. Pruden. Antibiotic Resistance Genes (ARG) as Emerging Environmental Contaminants. ASCE Environmental and Water Resources Institute (EWRI) World Environmental and Water Resources Conference, May 21-25, 2006, Omaha, NE.
  5. Storteboom, H., and A. Pruden. Fate of Antibiotic Resistance Genes (ARG) in the Environment in Response to Biological Treatment. 3 rd Annual WEF/AWWA Regional Student Conference, May 19 th, 2006, Colorado State University, Fort Collins, CO. ( won 3 rd prize for student oral presentations).
  6. Hong, H., and A. Pruden. Microbial Characterization of Sulfate-reducing Columns Remediating Acid Mine Drainage. 3 rd Annual WEF/AWWA Regional Student Conference, May 19 th, 2006, Colorado State University, Fort Collins, CO.
  7. Hiibel, S.R., Pereyra, L.P., Pruden, A., and K.F. Reardon. Spatial Variation of Microbial Communities in Two Field-Scale Sulfate-Reducing Permeable Reactive Zones Treating Mine Drainage. 35 th Annual Biochemical Engineering Symposium, South Dakota School of Mines and Technology, Rapid City, SD, April 29, 2006.
  8. Pruden, A., Pereyra, L.P., Hiibel, S., Reardon, K.F., and D. Reisman. Microbiology of Sulfate-Reducing Passive Treatment Systems. 7 th International Conference on Acid Rock Drainage (ICARD), March 26-29, 2006, St. Louis, MO.
  9. Hiibel, S.R., Pereyra, L.P., Pruden, A., and K.F. Reardon. Microbiological Comparison of Two Field-Scale Sulfate-Reducing Permeable Reactive Zones Treating Mine Drainage. 26th Annual American Geophysical Union Hydrology Days, March 20 - 22, 2006, Colorado State University, Fort Collins, CO.
  10. Sharma, A., Collins, G.J., Moore, C. Dense Medium Plasma: A Promising New Water Treatment Approach. 26th Annual American Geophysical Union Hydrology Days, March 20 - 22, 2006, Colorado State University, Fort Collins, CO.
  11. H.N. Storteboom, S.C. Kim, K.H. Carlson and A. Pruden. Antibiotic Resistance Genes (ARG) in the Environment: Effect of Composting. 26th Annual American Geophysical Union Hydrology Days, March 20 - 22, 2006, Colorado State University, Fort Collins, CO.
  12. Hanson, R.E., Sans, C., Hoelscher, M., Pruden, A., and K.F. Reardon. Using Molecular Tools to Monitor a Microbial Consortium Degrading a 12-Chemical Mixture. 26th Annual American Geophysical Union Hydrology Days, March 20 - 22, 2006, Colorado State University, Fort Collins, CO.  
  13. Raynal, M., Pruden, A., and T. Sale. Treatment of MTBE and BTEX at a Local Refinery: Bench and Field Studies   . 26th Annual American Geophysical Union Hydrology Days, March 20 - 22, 2006, Colorado State University, Fort Collins, CO.  
  14. Pei, R., Pruden, A., Kim, S-C, and K.H. Carlson. Antibiotic Resistance Genes (ARG) Studies in the Poudre River and Northern Colorado.   26th Annual American Geophysical Union Hydrology Days, March 20 - 22, 2006, Colorado State University, Fort Collins, CO.  
  15. Kidner, E.M., L.A. Roesner, L.A., and A. Pruden. Guidance for Improving Monitoring Methods for Stormwater-Borne Solids.   26th Annual American Geophysical Union Hydrology Days, March 20 - 22, 2006, Colorado State University, Fort Collins, CO.
  16. Sans, C., Pruden, A., Hoelscher, M.R. and K. F. Reardon. Microbial Analysis of an Aerobic Reactor Degrading Organic Pollutant Mixtures. 10 th Mediterranean Conference of Chemical Engineering, November 15-18, 2005, Barcelona, Spain.
  17. Pei, R., Huxford, K. and A. Pruden. Quantifying Antibiotic Resistance in the Sediments of a Mixed-Landscape River. Selected for Alternate Oral Presentation, Water Environment Federation Technical Conference and Exhibition, October 29 – Nov. 2, 2005, Washington D.C.
  18. Klein, D.A., R.M. Flores, S. McKinney, L.P. Pereyra, and A. Pruden. Cultural and Molecular Studies of Methanogens in Paleocene Fort Union Coals from the Powder River Basin of Wyoming and Montana. American Association of Petroleum Geologists (AAPG), September 24-27, 2005, Jackson, WY.
  19. Albertson, M.L., Pruden, A., and R.T. Oliver. Enhanced Anaerobic Digestion of Biomass Waste for Optimized Production of Renewable Energy and Solids for Compost. Greenhouse Gases and Animal Agriculture, ElSevier International Congress Series, September 20-24, 2005, Zurich.
  20. Pruden, A., Hong, H., Inman, L.Y., Logan, M.V., Sans, C., Ahmann, D., Figueroa, L.A. and K. F. Reardon. Microbial Characterization of Sulfate-Reducing Columns Remediating Acid Mine Drainage. American Society for Mining Reclamation (ASMR), June 18-23, 2005, Breckenridge, CO.
  21. Pereyra, L.P., Hanson, R.E., Hiibel, S., Pruden, A., and K.F. Reardon. Comparison of Inocula Applied in the Remediation of Acid Mine Drainage by Sulfate Reduction. American Society for Mining Reclamation (ASMR), June 18-23, 2005, Breckenridge, CO. ( Won 2 nd Prize in Student Paper Competition)
  22. Sharma, A., Pruden, A, and G.J. Collins. Open-air Bacterial Inactivation using a RF Powered Hollow-Slot Plasma Device. 32 nd IEEE Conference on Plasma Science (ICOPS), June 18 – 23, 2005, Monterey, CA.
  23. Pereyra, L.P., and A. Pruden. Comparison of Inocula Applied in the Remediation of Heavy Metals by Sulfate Reduction. WEF/AWWA Student Conference, May 18, 2005, University of Wyoming, Laramie, WY.
  24. Pei, R., and A. Pruden. Quantifying Antibiotic Resistance in the Sediments of a Mixed Landscape River. WEF/AWWA Student Conference, May 18, 2005, University of Wyoming, Laramie, WY.
  25. Hanson, R.E., and A. Pruden. Dynamics of a CSTR Consortium Degrading a 12- Chemical Mixture. WEF/AWWA Student Conference, May 18, 2005, University of Wyoming, Laramie, WY.
  26. Klein, D.A., R.M. Flores, L.P. Pereyra and A. Pruden. Putative Methanogen Molecular Evidence in Paleocene Fort Union Coals in the Powder River Basin, WY and MT. Geological Society of America Annual Meeting and Exposition, November 7-10, 2004, Denver, CO.
  27. Messner, N.L., L.P. Pereyra, A. Pruden, K.F. Reardon, and C. Sans-Mazón. Effect of inocula on Heavy Metal Removal by Sulfate-Reducing Columns. 11 th Annual Conference on Tailings and Mine Waste, October 10-13, 2004, Vail, CO. Balkema Publishers, Leiden, Netherlands, pp. 213-221.
  28. Pruden, A., and Pei, R. Microbial Community Dynamics of BTEX Inhibition of MTBE and TBA Biodegradation. 10th International Symposium on Microbial Ecology, August 22-27, 2004, Cancun, Mexico.
  29. Sharma, A., Pruden, A., Yu, Z., and G.J. Collins. Bacterial Deactivation in Open-Air by the Afterglow Plume Emitted from a Grounded Hollow Slot Electrode. 31 st IEEE Conference on Plasma Science (ICOPS), June 28 – July 1, 2004, Baltimore, MD.
  30. Hong, H., Pruden, A., Logan, M., Inman, L.Y., Sans, C., Reardon, K.F., and Figueroa, L.A. Microbial Analysis of Sulfate-Reducing Columns Remediating Heavy Metals. WEF/AWWA Student Conference, May 18, 2004, Colorado School of Mines, Golden, CO.
  31. Hong, H., Pruden, A., Inman, L., Walston, K., Logan, M., Reardon, K.F., and Figueroa, L. Molecular Characterization of Microbial Communities Present in Sulfate-Reducing Permeable Reactive Zones Remediating Heavy Metals. Mine Design, Operation, and Closure Conference, April 27-29, 2004, Polson, MT.
  32. Pei, R., Anurita, and Pruden, A. Microbial Community Dynamics of MTBE and BTEX Degradation. Hydrology Days, March 10-12, 2004, Colorado State University, Fort Collins, CO.
  33. Yu, Z., A. Pruden, and G. Collins. RF-Driven Dense Medium Plasma Reactor for Remediation of MTBE and TBA Contaminated Water, Petroleum Hydrocarbons and Organic Chemicals in Ground Water: Prevention, Assessment, and Remediation Conference & Exposition, Aug. 22, 2003, Costa Mesa, CA.
  34. Sedran, M.A., Suidan, M.T., Venosa, A.D., and Pruden, A. Analysis of an Aerobic Fluidized-Bed Reactor Degrading MTBE and BTEX at Reduced EBCTs. AWWA 2003 Annual Conference, June, 2003, Anaheim, CA.
  35. Pruden, A.,Sedran, M.A., Suidan, M.T., and A.D. Venosa.Biodegradation of MTBE and BTEX in an Aerobic Fluidized Bed Reactor. 3 rd Congress of the International Water Association, April, 2002, Melbourne, Australia.
  36. Garcia-Blanco, S., A. Pruden, M.T. Suidan, A.D. Venosa, and K. Lee. Study of Bioremediation Treatment Effects on Microbial Populations in a Crude Oil-Contaminated Coastal Marsh by Denaturing Gradient Gel Electrophoresis (DGGE). 3 rd Congress of the International Water Association, April, 2002, Melbourne, Australia.
  37. Pruden, A., M.A. Sedran., G.J. Wilson, M.T. Suidan, A.D. Venosa. Effect of BTEX on the Degradation of MTBE and TBA by a Mixed Bacterial Consortium. American Chemical Society 222 nd National Meeting, August 26-30, 2001, Chicago, IL.
  38. Pruden, A., M.T. Suidan, A.D. Venosa, and J.R. Morrison. Denaturing Gradient Gel Electrophoresis Analysis of a Methyl tert-Butyl Ether Degrading Culture Applied in a Membrane Bioreactor. 2 nd Congress of the International Water Association, October, 2001, Berlin, Germany.
  39. Morrison, J.R., M.T. Suidan, A.D. Venosa, and A. Pruden. Use of a Membrane Bioreactor for Removal of MTBE. WEFTEC, October, 2001, Atlanta, GA.
  40. Sedran, M.A., A. Pruden, G.J. Wilson, M.T. Suidan, and A.D. Venosa. Biodegradation of MTBE in the Presence of BTEX Under Batch and Continuous Feed Conditions. WEFTEC, October, 2001, Atlanta, GA.
  41. Pruden, A., G.J. Wilson, M.T. Suidan, A.D. Venosa, and M.S. Sedran. Characterization and Degradation Kinetics of Aerobic MTBE Degrading Cultures, In: Proceedings of Battelle 6 th International Symposium on In Situ and On-Site Bioremediation, June, 2001, San Diego CA.
  42. Pruden, A., G.J. Wilson, M.T. Suidan, and A.D. Venosa. Identification and Monitoring of MTBE degrading cultures by DGGE analysis. In: Proceedings of Research Symposium. WEFTEC, October, 2000, Anaheim, CA.
  43. Wilson, G.J., A. Pruden, M.T. Suidan, and A.D. Venosa. Mineralization of MTBE with Various Primary Substrates. In Proceedings of Battelle 2 nd International Conference on Remediation of Chlorinated and Recalcitrant Compounds, May 2000, Monterey, CA.
  44. Wilson, G.J., A. Pruden, M.T. Suidan, and A.D. Venosa. Aerobic Biodegradation of Gasoline Oxygenates MTBE and TBA. In Proceedings of 1 st Congress of the International Water Association, July, 2000, Paris, France.

 

Abstracts and Poster Presentations

  1. Pruden, A., Hiibel, S.R., Pereyra, L.P., Figueroa, L.A., and D. Reisman. Microbiology of Field Sulfate-Reducing Bioreactors Treating Mining Influenced Water. Hard Rock Mining Conference, Nov. 14-16, Tucson, AZ.
  2. Hiibel, S.R., Pruden, A., and K.F. Reardon. Structure and Activity of a Microbial Community Treating Acid Mine Drainage. Submitted to: 2006 Annual AIChE Meeting, Nov. 12-17, 2006, San Francisco, CA.
  3. Pereyra, L.P., and A. Pruden. Comparison of Inocula Applied in the Remediation of Heavy Metals by Sulfate Reduction. 3 rd Annual WEF/AWWA Regional Student Conference, May 19 th, 2006, Colorado State University, Fort Collins, CO. ( won 1 stprize for student poster presentations).
  4. Hiibel, S.R., Pruden, A., and K.F. Reardon. Acid Mine Drainage Remediation Using Sulfate-Reducing Permeable Reactive Zones: A Two Field-Site Microbiological Comparison. 3 rd Annual WEF/AWWA Regional Student Conference, May 19 th, 2006, Colorado State University, Fort Collins, CO.
  5. Raynal, M. and A. Pruden. Treatment of MTBE and BTEX at a Local Refinery: Bench and Field Studies. 3 rd Annual WEF/AWWA Regional Student Conference, May 19 th, 2006, Colorado State University, Fort Collins, CO.
  6. Blanchard, K., and A. Pruden. Characterization of Microorganisms in Sulfate-Reducing Permeable Reactive Zones. Poster presentation at Undergraduate Research and Creativity Showcase, April 18 th 2006, Colorado State University.
  7. Storteboom, H., and A. Pruden. Investigation of Composting as a Means of Minimizing the Impact of Agricultural Antibiotics in the Environment. WEF/AWWA Student Conference, May 18, 2005, University of Wyoming, Laramie, WY.
  8. Kashani, N., and A. Pruden. Comparison of Sulfate-Reducing Inocula in the Remediation of Acid Mine Drainage. Poster presentation at Undergraduate Research and Creativity Showcase, April 19 th 2005, Colorado State University.
  9. Gabbert, K., D.A. Klein, and A. Pruden. Evidence for the Presence of Methanogen DNA in Paleocene Low-Rank Coal Beds. Poster presentation at Undergraduate Research and Creativity Showcase, April 19 th 2005, Colorado State University.
  10. Pruden, A., Hong, H., Logan, M., Inman, L., Sans Mazón, C., Reardon, K.F. and Figueroa, L.A. Microbial community dynamics of sulfate-reducing permeable reactive zones remediating heavy metals. 10th International Symposium on Microbial Ecology, August 22-27, 2004, Cancun, Mexico. p. 229.
  11. Pereyra, L.P., Hanson, R.E., Messner, N.L., Pruden, A., and K.F. Reardon. The Optimization of Inocula for the Bioremediation of Acid Mine Drainage Using Sulfate Reduction, HSRC Annual Meeting, August 8-10, 2004, Colorado State University, Fort Collins, CO.
  12. Messner, N.L., H. Hong, L.Y. Inman, K.R. Walston, A. Pruden, N.M. DuTeau, K.F. Reardon M. Logan, D. Ahmann, and L. Figueroa. Microbial Population Dynamics in Sulfate-Reducing Mine Drainage Treatment Systems. Battelle Conference on Remediation of Chlorinated and Recalcitrant Compounds, May 24-27, 2004, Monterey, CA. p. E2.
  13. Pei, R., Anurita, and A. Pruden. Microbial Community Dynamics During BTEX Inhibition of MTBE and TBA Biodegradation. WEF/AWWA Student Conference, May 18, 2004, Colorado School of Mines, Golden, CO.
  14. Messner, N.L., Pereyra, L., Sans, C., Pruden, A., Reardon, K.F. Effect of Innocula on Heavy Metal Removal by Sulfate-Reducing Columns. WEF/AWWA Student Conference, May 18, 2004, Colorado School of Mines, Golden, CO ( won 2 nd prize in poster competition).
  15. Messner, N.L., Lucchesi, K., Pruden, A., and K.F. Reardon. Poster presentation at Colorado State University Engineering Days, April 14 th ( won 3 rd prize for Senior-level competition), Colorado State University, April, 2004.
  16. Messner, N.L., Pruden, A., Hong, H., Logan, M., Reardon, K., and L. Figueroa. Microbial Community Analysis of Passive Bioreactors for Remediation of Heavy Metals Emanating from Acid Mine Drainage. Poster presentation at Colorado State University Engineering Days, April 11 th ( won 1st prize for Junior-level competition), Colorado State University, April, 2003.
  17. Hong, H., Messner, N.L., Logan, M., Reardon, K.F., Figueroa, L., and D. Ahmann. Denaturing Gradient Gel Electrophoresis (DGGE) of Microbial Communities Mediating Remediation of Mine Drainage. Poster presentation, HSRC annual meeting, Colorado School of Mines, May 19 th, 2003.
  18. Pruden, A. and M.T. Suidan. Biodegradation of Methyl tert-Butyl Ether by Pure Bacterial Culture UC1. 103 rd Annual American Society for Microbiology General Meeting, May, 2003, Washington, D.C.
  19. Pruden, A., Y-J Chang, J. Brüggemann, J.R. Stephen, S.J. Macnaughton, A.D. Venosa and D.C. White. 16S rDNA Analysis of Aerobic Enrichment Cultures of Freshwater and Marine Hydrocarbon Degrading Consortia and Effect of PCR Primer Choice. 99 th Annual ASM General Meeting. May 30- June 3, 1999, Chicago, IL.
  20. Pruden, A., and G.W. Uetz. Assessment of Predation Cost of Male Decoration and Courtship Displays in Wolf Spiders Using Video Imaging. Annual Meeting of Animal Behavior Society, June, 1997, College Park, MD.
  21. Orr, M.A., A. Pruden, and G.W. Uetz. Divergence in Multi-Modal Communication in Two Wolf Spider Species. Annual Meeting of Animal Behavior Society, June, 1997, College Park, MD.
  22. Uetz, G.W., M.A. Orr, A. Pruden, R. Mendoza, and B. Kilink. Male Condition, Courtship Vigor and Mate Choice in Wolf Spiders. Annual Meeting of Animal Behavior Society, June, 1997, College Park, MD.

 

TEACHING & MENTORING SUMMARY

 Overview

Integrating research and education and promoting diversity have been major themes in teaching and mentoring. Courses taught (summarized below) emphasize the interdisciplinary nature of Environmental Engineering. New courses (CE 540, CE 580, and CE 536) have been developed that integrate biotechnology and Environmental Engineering, including a new laboratory-based hands-on course, CE 580 Biomolecular Tools for Engineers. This course has received support from NSF through a Course Curriculum and Laboratory Improvement (CCLI) program through which its content and delivery is being developed in collaboration with Environmental Engineering faculty at four other universities (University of Cincinnati, Pennsylvania State University, Harrisburg, University of Wisconsin, Milwaukee, and University of South Florida). A text book, entitled Molecular Biology for Environmental Engineers, is being prepared through support of this grant and Taylor and Francis has already committed to its publication.

 

In order to provide an overarching structure to the research mentoring activities in the Pruden lab, the “MERGE” (Mentoring Environmental Research in Genomics and Engineering) program has recently been launched. To date, ten undergraduates and nine graduate students have participated in the program. The overall goal of the program is to help develop leadership skills in engineering research and to promote diversity, both in terms of undergraduate education (Biochemistry, Biology, Electrical Engineering, Chemical Engineering, Civil & Environmental Engineering, etc.) and underrepresented groups (currently ~50% female). Funding has been provided directly by National Science Foundation and the Colorado Institute of Technology, and indirectly through various programs (CO-AMP, NSF REU, McNair, and currently funded research grants).

 

Courses Taught

  • CE 580 Molecular Biology for Engineers, 3 cr. w/lab (2005, 2006)
  • EV 441 Water-Wastewater Characterization, 1 cr. lab (2005, 2006)
  • CE/CH 540 Fundamentals of Environmental Biotechnology, 2 cr. lecture (2005, 2006)
  • CE 536 Wastewater Treatment, 1 cr. lecture (2006)
  • CE 108 Civil Engineering Principles I –team, 3 cr. w/ lab (2004, 2005, 2006)
  • CE 581 Water Engineering for International Development, 3 cr. lecture (5 week sanitation section, 2004, 2006)
  • CE 540 Treatment of Contaminants I, 3 cr. Lecture (2002, 2003)
  • CE 538 Aquatic Chemistry, 3 cr. lecture (first 8 weeks 2003)
  • CE 742 Advanced Topics in Environmental Engineering: Biomolecular Tools for Environmental Engineers, 3 cr. lecture w/ lab (2003)

 

Funded Projects with Educational Component

 

CAREER: Antibiotic Resistance Genes (ARG) as Emerging Pollutants in Our Water: Pathways, Mitigation, and Treatment, National Science Foundation, Feb. 1, 2006- Jan. 31, 2010. $400,000. P.I.

Molecular Biology for Environmental Engineers, National Science Foundation: Course Curriculum and Laboratory Improvement (CCLI), Aug. 1, 2005 – July 31, 2007. $40,000. P.I.

Environmental Biotechnology for Engineers in the 21 st Century, Colorado Institute of Technology, Oct. 26, 2004-May. 25 2006. $100,000. P.I., Co-P.I.s: Kenneth F. Reardon and George J. Collins.

 

Educational Publications

  1. Oerther,D.B., Maltbie, C., Li, B., Li, J., Pruden, A., and P. Stroot. Disseminating Molecular Biology for Environmental Engineers with NSF CCLI Support. American Society of Engineering Education (ASEE) Annual Conference, June 18-21, 2006, Chicago, IL.
  2. Kaminski, K. and Pruden, A. Environmental Engineering Keeps Pace with Advances in Molecular Biology and Biotechnology: Integrated Education and Research. American Educational Research Association (AERA), April 6-11, 2006, San Francisco, CA.
  3. Siller, T.J., Pruden, A., and Johnson, G. A Baccalaureate in International Engineering. International Conference on Educational Technology, ICET, October 26, 2005, Budapest, Hungary.

Advising Graduate and Undergraduate Research

Past and Current Graduate Students (Ph.D.):

  1. Ruoting Pei“Antibiotic resistance genes (ARG) as environmental contaminants: Occurrence in Cache-La Poudre River Watershed and Response to Biological Treatment” Final Defense set for August 23, 2006, graduation: December, 2006.
    Two peer- reviewed publications accepted (Water Research and ES&T)
    Has accepted a Post-Doc position at Duke University
    , starting Sept. 1, 2006.
  1. Ashish Sharma (Co-advised with George J. Collins, Electrical Engineering)
    “Microbial Inactivation Using a Hollow-Slot RF Electrode Operated at 60 mHz”
    Anticipated graduation: May 2007
    Three peer-reviewed publications in print/accepted (ES&T, J. Phys. D, and IEEE)
  1. Maria Raynal “In-Situ Wetland Treatment of MTBE and BTEX”
    Anticipated graduation: August 2007
  2. Luciana Pereyra “Development of Quantitative Tools Targeting Functional Genes to Guide Design and Operation of Passive Mine Drainage Treatment Systems”  
    Anticipated graduation: May 2008
    One peer-reviewed publication submitted to Water Research.
  3. Sage Hiibel (Co-advised with Kenneth F. Reardon, Chem. & Biol. Engineering) “Optimization of Microbial Inocula for High Performance Passive Remediation of Acid Mine Drainage”
    Anticipated graduation: May 2008
    One peer-reviewed publication submitted to Water Research.

 

Past and Current Graduate Students (MS):

  1. HyunSuk Hong (MS) “Application of Biomolecular Tools for Characterization of Microbial Communities in Columns Simulating Sulfate-Reducing Permeable Reactive Zones Remediating Mine Drainage”
    Passed Final Defense, July 7 th, 2006, graduation: August, 2006.
    Has accepted a Ph.D. student position at NC State University.
  2. Heather Storteboom (MS) “Monitoring Response of Tetracycline Resistance Genes tet(W) and tet(O) to Composting and Stockpiling of Antibiotic-Containing Manures”
    Passed Defense Date July 6 th, 2006, graduation: August, 2006.
    Has accepted a Ph.D. student position at Colorado State University.
  1. Rachel Hanson (MS) “Monitoring Microbial Community Diversity During Biodegradation of Contaminant Mixtures”
    Anticipated graduation: December 2006
     
  2. Matthew Hewitt (MS) “Developing Tools for On-line Biosecurity Monitoring of Water Distribution Systems”
    Anticipated graduation: December 2007

Undergraduate Research Advisees (program, graduation date, post-grad sponsor):

Nicole Messner (Chem. Engg., 2003, JM Hyde Associates, Philadelphia, PA)
Katherine Huxford (Chem. Engg., 2004, National Starch and Chem. Co., Chicago, IL)
Nella Kashani (Chem. Engg., 2005, TriHydro Environmental, Laramie, WY)
Kendra Gabbert (Civil Engg., 2006, Felsburg, Holt & Ullevig, Denver, CO)
April Tischer (Env. Engg., 2006, Research Associate, Pruden Lab)
Ashley Sicks (Microbiology, 2006, Amgen Corp., Longmont, CO)
Katie Lucchesi (Civil Engg., expected 2007)
Kaileen Blanchard (Chem. Engg., expected 2007)
Cristophe Venot (Microbiology, expected 2007)
Maria Virginia Gibbons (Env. Engg., expected 2008)

Committee Member:

Ph.D. (program, graduation date, post-grad sponsor):

Durmus Cesur (Env. Engg., 2004, San Antonio River Authority, San Antonio, TX)
Ninad Gujarathi (Chem. Engg., 2005, Asst. Prof., Chem. Engg., Maharashtra Academy of Engineering, India)
Sung-Chul Kim (Env. Engg., defense date set for August, 2006)
Jongmun Cha (Env. Engg., expected Spring 2007)
Mary Beth Talty (Env. Engg., expected Spring 2007)
Ramadan Alkhatib (Civil Engg., expected Spring 2007)
Matt Hoelscher (Chem. Engg., expected Fall 2007)
Ravi Rahul (Elec. Engg., expected Fall 2007)
Seong-Ho Cho (Env. Engg., expected Spring 2008)
Carla Lacerda (Chem. Engg., expected Spring 2008)
Casey Boczon (Biology, expected Spring 2008)

 

M.S. Plan A (program, graduation date, post-grad sponsor):

Ashraf Al-Zaghal (Env. Engg., 2003, Palistinian Government, Palestine)
Ashish Sharma (Electrical Engg., 2004, Ph.D. Student, CSU)
Amy Zimmerman (Env. Engg., 2006, Carollo Engineers, Littleton, CO
)
Elizabeth Kidner (Env. Engg., expected 2007)

M.S. Plan B (program, graduation date, post-grad sponsor):

Mohammed Alhajri (Environ. Engg., 2003, Saudi Arabian Government)
Ninad Gujarathi (Chem. Engg, 2003, Ph.D. student ChemE., CSU)
Michael Giardullo (Civil Engg., 2006, Weiler Engineering Corp., Port Charlotte, FL )
Claudia Romero (Education, 2006, Instructor, Front Range Community College)

 

Visiting Professors:

  • Kyung-Ha Yoon, Soonchunhyang University, Korea, 2006- 2007
  • Catherine Biggs, University of Sheffield, U.K., August, 2005
  • Audra Morse, Texas Tech University, August, 2005
  • Carmen Sans, University of Barcelona, Spain, 2003-2004
  • Carmen Gonzalez, University of Barcelona, Spain, June-Aug., 2004
  • Santiago Esplugas, University of Barcelona, Spain, June-Aug., 2004  

 

 

SERVICE & OUTREACH SUMMARY

 

Memberships

International Society for Microbial Ecology        

American Society for Microbiology            

Society for Environmental Toxicology & Chemistry  

Association of Environmental Engineering and Science Professors (AEESP)

American Society of Engineering Education (ASEE)

Water Environment Federation

International Water Association

American Chemical Society

 

Professional Service

American Water Works Association (AWWA) Project Advisory Committee (PAC), Synthesis Document on the State of Science of Molecular Techniques for Application to the Drinking Water Industry, 2005-2006.

Water Environment Federation (WEF) Groundwater Committee, WEFTEC 2007 session organizational team, 2006-present.

Organized Hydrology Days Session “Emerging Contaminants, Emerging Technologies” April 21, 2006.

7 th International Conference on Acid Rock Drainage (ICARD), Conference Proceedings Reviewer.

Organized Lecture series on “Biomolecular Tools in Environmental Engineering,” Including: Daniel B. Oerther, University of Cincinnati; Peter Stroot, University of South Florida; Baikun Li, Pennsylvania State University, Harrisburg; and Jin Li, University of Wisconsin, Milwaukee. Colorado State University, October 11 th, 2005.

Organized Summer Lectures: Catherine Biggs, University of Sheffield, U.K., and Audra Morse, Texas Tech Universtiy, TX. Colorado State University, August 16 and September 1, 2005.

Organized Bruce Rittmann Association of Environmental Engineering & Science Professors (AEESP) Distinguished lecture, Colorado State University, October 11, 2004.

American Petroleum Society, Petroleum Research Fund, Ad-Hoc Proposal Reviewer, Aug., 2004.

NSF Panel Member, July, 2004.

Participant in NSF sponsored Women in Engineering Leadership Institute (WELI), Salt Lake City, Utah, Nov. 5-8, 2003.

Participant in NSF sponsored Share the Future Conference, Phoenix, AZ, March 15-16, 2003.

 

Reviewer (# reviewed since 2002)

  • Biodegradation (1)
  • Bioremediation Journal (2)
  • Bioresource Technology (2)
  • Biotechnology and Bioengineering (2)
  • Environmental Science and Technology (2)
  • Environmental Toxicology and Chemistry (12)
  • IEEE Transactions on Plasma Science(2)
  • Journal of Applied Microbiology (4)
  • Journal of Hazardous Materials (2)
  • Water Environment Research (1)
  • Water Research (1)

 

Student Service

Faculty Advisor to organization of the WEF/AWWA 3 rd Annual Student Conference, May 19 th, 2006, hosted by Colorado State University (six participating universities and ten corporate sponsors).

Faculty Advisor to the ASCE Water Treatment from your Kitchen and Beyond Regional Student Competition, April 8 th, 2006, Stockton, CA ( won 3 rd Prize).

Faculty Advisor to the Environmental Engineering Society (EES), January 2006-present.

NSF Research Experience for Undergraduates (REU) Faculty Mentor, Summer 2003, 2004, and 2006.

Judge, Undergraduate Research and Creativity Showcase, Spring 2004, 2005, and 2006.

Volunteer, Ram Welcome, Fall, 2005.

Panel Speaker at Society of Women Engineers (SWE) meeting, February, Nov. 15, 2005.

Student Leaders in Engineering (SLE) Faculty Mentor, 2003-2005.

Judge, College of Engineering Silver Medal Award, Spring 2003.

Volunteer, Annual Engineering Exploration Day, Fall 2002, 2004, 2005 and Spring 2005.

Saturday Morning Engineering Program Volunteer, Engineering Education for girls K-12, 2003 and 2004.

Judge, Society for Women Engineers (SWE) Sled-building contest, Feb. 2003.

 

University, College, and Departmental Service

CSU International Development Studies Board, member (2003-2004), chair (2005-2006).

Departmental Advisory Board, 2005- present.

Environmental Engineering Graduate Program Committee, 2004-present.

Dean of the College of Engineering Search Committee, 2004-2005.

CE Faculty Search Committee (Geotechnical), 2003-3004.

CE Faculty Search Committee (Water), 2002-2003.

CE Computer Committee, 2002-2003.

 

Publicity

Interviewed for Loos Tales Radio Show, Nov. 15, 2005.

Quoted in Coloradoan article, Livestock Antibiotics Discovered in Poudre, Oct. 23, 2004.

Quoted in Denver Post article, Animal Drugs May Taint Water, Oct. 20, 2004.

Feature Article in CSU Engineering Review Magazine, vol. 13, p. 7, 2004.

Feature Interview in CSU College of Engineering DVD, 2003.