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| I. |
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Basics of Microbiology (Chapter 1) - 2 weeks |
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a. Physiology of the cell |
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b. Taxonomy and Phylogeny |
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i. |
Eukaryotes and Prokaryotes (Bacteria and Archaea), viruses |
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ii. |
Morphology: cocci, bacillus, spirillum, filamentous |
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iii. |
Trophic classifications: hetero/auto/chemo/litho/organotrophs |
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c. Biochemistry and Enzymes |
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i. |
Michaelis-Menten kinetics |
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ii. |
Enzyme regulation |
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d. Metabolism - energy balance |
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i. |
Anabolism/catabolism |
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ii. |
Krebs Cycle/ ATP production |
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e. Molecular Biology - DNA/RNA |
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i. |
Enzyme production and regulation |
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ii. |
Plasmids |
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iii. |
Molecular tools for identifying and enumerating bacteria |
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f. Microbial ecology |
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i. |
Selection |
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ii. |
Exchange of genetic info. |
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iii. |
Adaptation |
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iv. |
Enrichment cultures |
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| II. |
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Stoichiometry and Bacterial Energetics (Chapter 2) - 2 weeks |
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a. Mass balances |
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b. Redox reactions: electron donor/electron acceptor |
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i. |
Redox half-reactions |
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c. Energy balances (delta G) |
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d. Biological Growth |
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i. |
Substrate Partitioning and Theoretical Yield |
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ii. |
Electron acceptors, fermentation |
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iii. |
Nitrogen source |
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e. BOD/COD |
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| III. |
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Microbial Kinetics (Chapter 3) - 2 weeks |
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a. Monod Kinetics |
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i. |
Determination of rate constants |
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ii. |
No growth |
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iii. |
With growth |
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b. Basic Mass balance (dX/dt; dS/dt) |
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c. Enzyme inhibition - Haldane Kinetics |
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d. Autocatalytic reactions |
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e. Alternate Rate Expressions |
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i. |
Logistic growth |
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ii. |
Logarithmic growth |
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| IV. |
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Reactors (Chapter 5) - 2 weeks |
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a. Basic Reactors |
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i. |
Batch |
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ii. |
CSTR |
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iii. |
Plug-Flow |
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b. Biofilm Reactors |
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i. |
Packed Bed |
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ii. |
Fluidized Bed |
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iii. |
Rotating Biological Contactor |
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c. Reactor Arrangements |
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i. |
Recycle |
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ii. |
Series |
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iii. |
Parallel |
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iv. |
Hybrid |
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v. |
Sequencing Batch |
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d. Basic mass balances and design considerations of each reactor type |
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| V. |
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The Activated Sludge Process (Chapter 6) - 2 weeks |
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a. Characteristics of Process Design |
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b. Process Configurations |
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i. |
Oxygen supply |
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ii. |
Loading modifications |
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c. Design and Operating Criteria |
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i. |
F/M ratio |
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ii. |
SRT |
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iii. |
MLSS and recycle ratio |
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d. Aeration |
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i. |
Oxygen transfer rates |
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ii. |
Diffuse/ mechanical aeration systems |
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e. Sludge-settling problems (bulking, foaming, etc.) |
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f. Analysis and design of settling |
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i. |
Type I, II, III "zone settling" (M&E chapter 6) |
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ii. |
Loading criteria |
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iii. |
Basics of flux theory |
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| VI. |
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Aerobic Biofilm Processes (Chapter 8, also Chapter 4 "Biofilm Kinetics") - 2 weeks |
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a. Properties of microbial aggregation |
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b. Idealized biofilm |
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c. Estimating kinetic parameters in a biofilm |
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d. SRT in a biofilm |
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e. Biofilm process considerations |
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f. Trickling filters/ biological towers |
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g. RBCs |
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h. Fluidized-bed reactors |
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| VII. |
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Nutrient Removal (Chapters 9 "Nitrification," 10 "Denitrification," and 11 "Phosphorus Removal") - 2 weeks |
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a. Nitrification |
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i. |
Biochem and physiology of nitrifying bacteria |
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ii. |
Activated Sludge versus Biofilm nitrification |
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iii. |
Role of BODL : TKN ratio |
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b. Denitrification |
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i. |
Physiology of denitrifying bacteria |
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ii. |
Tertiary denitrification |
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iii. |
One-Sludge Denitrification |
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c. Phosphorous Removal |
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i. |
Phosphate uptake (normal and luxury uptake) |
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ii. |
Precipitation by metal-salts addition |
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iii. |
Enhanced biological P removal, and Barden-Pho process |
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| VIII. |
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Anaerobic Treatment (Chapter 13) - 1 week |
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a. Applications of anaerobic (methanogenic) treatment |
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b. Reactor configurations |
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c. Chemistry and microbiology |
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d. Kinetics of methanogenesis |
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i. |
Temperature |
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ii. |
CSTR |
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iii. |
Complex substrates |
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iv. |
Optimization of anaerobic digestion |
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e. Special factors for design consideration |
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i. |
Loading criteria |
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ii. |
Mixing |
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iii. |
Heating |
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iv. |
Gas collection |
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v. |
Performance |
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| IX. |
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Detoxification of Hazardous Chemicals (Chapter 14) - 1 weeks |
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a. Factors causing molecular recalcitrance |
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b. Classes of synthetic organics |
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c. Energy metabolism vs. cometabolism |
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d. Electron donor/acceptor |
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e. Smin |
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f. Examples of biodegradation of common contaminants |