Dr. Susan K De Long

• Kim, S., Rossmassler, K., Broeckling, C.D., Galloway, S., Prenni, J. and De Long, S.K. Impact of inoculum sources on biotransformation of pharmaceuticals and personal care products. Water Research, in press
• Wilson, L.P., Sharvelle, S.E., De Long, S.K. (2016) Enhanced anaerobic digestion performance via combined solids- and leachate-based hydrolysis reactor inoculation. Bioresource Technology 220, 94-103. (Impact Factor: 6.102, Citations: 1)
• Ekeren, K.M., Hodgson, B., Sharvelle, S.E., De Long, S.K. (2016) Investigation of pathogen disinfection and regrowth in a simple graywater recycling system for toilet flushing. Desalination and Water Treatment 57(54), 26174-26186. (Impact Factor: 1.643)
• Irianni-Renno, M., Akhbari, D., **Olson, M.R., Byrne, A.P., Lefevre, E., Zimbron, J., Lyverse, M., Sale, T.C., De Long, S.K. (2016) Comparison of bacterial and archaeal communities in depth-resolved zones in an LNAPL body. Applied Microbiology and Biotechnology 100(7):3347-60. (Impact Factor: 3.716)
• Keely, S.P., Brinkman, N.E., Zimmerman, B.D., Wendell, D., Ekeren, K.M., De Long, S.K., Sharvelle, S., Garland, J.L. (2015) Characterization of the relative importance of human- and infrastructure-associated bacteria in grey water: a case study. Journal of Applied Microbiology 119, 289-301. (Impact Factor: 2.619, Citations: 6)
• Zeman, N.R., Irianni Renno, M., Olson, M.R., Wilson, L.P., Sale, T.C., De Long, S.K. (2014) Temperature impacts on anaerobic biotransformation of LNAPL and concurrent shifts in microbial community structure. Biodegradation.25, 569-585.
• Wilson, L.P., Loetscher, L.H., Sharvelle, S.E., De Long, S.K. (2013) Microbial community acclimation enhances waste hydrolysis rates under elevated ammonia and salinity conditions. Bioresour. Technol. 146, 15-22.
• Lefèvre, E., Pereyra, L.P., Hiibel, S.R., Perrault, E.M., De Long, S.K., Reardon, K.F., Pruden, A. (2013) Molecular assessment of the sensitivity of sulfate-reducing microbial communities remediating mine drainage to aerobic stress. Water Res. 47, 5316-25.
• Ledeker, B.M., De Long, S.K. (2013) The effect of multiple primer-template mismatches on quantitative PCR accuracy and development of a multi-primer set assay for accurate quantification of pcrA sequence variants. J. Microbiol. Methods. 3, 224-31.
• De Long, S.K., Li, X., Bae, S., Brown, J.C., Raskin, L., Kinney, K.A., Kirisits, M.J. (2012) Quantification of genes and gene transcripts for microbial perchlorate reduction in fixed-bed bioreactors. J. Appl. Microbiol. 112, 579-592.
• London, M.R., De Long, S.K., Strahota, M.D., Katz, L.E. and Speitel, G.E. (2011) Autohydrogenotrophic perchlorate reduction kinetics of a microbial consortium in the presence and absence of nitrate. Water Res 45, 6593-6601.
• Fagerstone, K.D., Quinn, J.C., Bradley, T.H., De Long, S.K., Marchese, A.J. (2011) Quantification of direct nitrous oxide emissions from microalgae cultivation. Environ. Sci. Technol. 45, 9449-9456.
• De Long, S.K., Kinney, K.A., Kirisits, M.J. (2010) qPCR assays to quantify genes and gene expression associated with microbial perchlorate reduction. J. Microbiol. Methods 83:270-4.
• Starkey, M., Hickman, J. H., Ma, L. Y., Zhang, N., De Long, S., Hinz, A., Palacios, S., Manoil, C., Kirisits, M.J., Starner, T. D. , Wozniak, D. J. , Harwood, C. S. and Parsek, M. R. (2009) Pseudomonas aeruginosa rugose small-colony variants have adaptations that likely promote persistence in the cystic fibrosis lung. J. Bacteriol. 191:3492-3503.
• De Long, S.K., Kinney, K.A., Kirisits, M.J. (2008) A targeted method to sequence prokaryotic biodegradation genes: prokaryotic SSH PCR cDNA subtraction. Appl. Environ. Microbiol. Jan;74(1):225-232.
• Teitzel, G.M., Geddie, A., De Long, S.K., Kirisits, M.J., Whiteley, M., Parsek, M.R. (2006) Survival and growth in the presence of elevated copper: transcriptional profiling of copper-stressed Pseudomonas aeruginosa. J. Bacteriol. Oct;188(20):7242-7256.
• Fanton, C.P., Rowe, M.W., Moler, E.J, Ison-Dugenny, M., De Long, S.K., Rendahl, K., Shao, Y., Slabiak, T., Gesner, T.G., MacKichan, M.L. (2006) Development of a screening assay for surrogate markers of Chk1 inhibitor-induced cell cycle release. J. Biomol. Screen. Oct;11(7):792-806.
• Holmes, L.E., Campbell, S.G., De Long, S.K., Sachs, A.B., Ashe, M.P. (2004) Loss of translational control in yeast compromised for the major mRNA decay pathway. Mol Cell Biol. Apr;24(7):2998-3010.
• Ashe, M.P., Slaven, J.W., De Long, S.K., Ibrahimo, S., Sachs, A.B. (2001) A novel eIF2B-dependent mechanism of translational control in yeast as a response to fusel alcohols. EMBO J. Nov 15;20(22):6464-6474.
• Ashe, M.P., Slaven, J.W., De Long, S.K., Ibrahimo, S. and Sachs, A.B. (2001) Fusel alcohols inhibit translation via a novel eIF2B-dependent control mechanism. Yeast 18, S74-S74.
• Ashe, M.P., De Long, S.K., Sachs, A.B. (2000) Glucose depletion rapidly inhibits translation initiation in yeast. Mol Biol Cell. Mar;11(3):833-848.

• Rossmassler, K., De Long, S.K. Meta-omics enabled approaches for identifying biomarkers directly from mixed microbial communities. 4th International Symposium on Bioremediation and Sustainable Environmental Technologies, Miami, FL, May, 2017.
• De Long, S.K., Sharvelle, S.E., Wilson, L.P., Lucas Loetscher. Advancing multi-stage anaerobic digestion technologies through improved hydrolysis processes. Global Waste Management Symposium, Palm Springs, CA, Jan 31-Feb 2, 2016.
• De Long, S.K., Wilson, L.P., Sharvelle, S.E. Advancing multi-stage anaerobic digestion technologies through improved hydrolysis processes. Environmental Research and Education Foundation Regional Summit, San Jose, CA, July 16-17, 2015.
• Ekeren, K.M., Sharvelle, S.E., De Long, S.K. Disinfection and regrowth of model pathogens in a graywater reuse treatment system. 19th Annual Water Reuse & Desalination Research Conference, Huntington Beach, CA, May 4-5, 2015.
• Zimmerman, B.D., Brinkman, N.E., Keely, S.P., Wiles, K.M., De Long, S.K., Sharvelle, S.E., Garland, J.L. Next generation sequencing reveals potential surrogates for performance monitoring of graywater recycling systems. 19th Annual Water Reuse & Desalination Research Conference, Huntington Beach, CA, May 4-5, 2015.
• Griffin, L.P., Sharvelle, S.E., De Long, S.K. "Advancing Multi-stage Anaerobic Digestion Technologies through Improved Hydrolysis Seeding Methods." Platform Presentation, Global Waste Management Symposium, Orlando, Florida, June, 2014.
• Griffin, L.P., Sharvelle, S.E., De Long, S.K. "The Impact of Process Conditions on Hydrolysis Efficiency and Methane Generation" Platform Presentation, Global Waste Management Symposium, Phenoix, Arizona, October, 2012.
• Irianni Renno, M., De Long, S.K., Zeman, N.R., Sale, T. "Microbially Mediated Depletion of Benzene in NAPL Impacted Soils" Platform Presentation, University Consortium for Field-Focused Groundwater Contamination Research, University of Guelph, Ontario, June, 2012.
• Griffin, L.P., Sharvelle, S.E., De Long, S.K. "Anaerobic Digestion of Organic Wastes: The Impact of Process Conditions on Hydrolysis Efficiency and Methane Generation" Oral presentation, International Biomass Conference and Expo, Denver, CO, April, 2012.
• Griffin, L.P., Sharvelle, S.E., De Long, S.K. "Anaerobic Digestion of Organic Solid Wastes: The Impact of Process Conditions" Oral presentation, Environmental Research and Education Foundation, Regional Summit, Austin, Texas, March, 2012.
• Griffin, L.P., De Long, S.K., Sharvelle, S.E. "The Impact of Operating Conditions on Hydrolysis Efficiency and Methane Generation for Anaerobic Digestion of Manure and Food Waste" Biocycle Global, San Diego, CA, April, 2011.
• De Long, S.K., K.A. Kinney, M.J. Kirisits. "Quantitative PCR Assays for Biological Perchlorate Treatment" Platform Presentation, American Water Works Association Annual Conference and Exposition, San Diego, California, June 2009.
• De Long, S.K., M.J. Kirisits, K.A. Kinney. "Perchlorate-Reducing Gene Targets for Bioremediation Applications" Platform Presentation, Battelle Remediation of Chlorinated and Recalcitrant Compounds Conference, Monterey, California, May 2008.
• Kirisits, M.J., S.K. De Long, K.A. Kinney, S.A. Desai, J.C. Brown. "Characterizing the Microbial Community in a Bioreactor Treating Perchlorate-Contaminated Groundwater" Platform Presentation, AWWA Inorganic Contaminants Workshop, Albuquerque, New Mexico, January 2008.
• De Long, S.K., M.J. Kirisits, K.A. Kinney. "Development of a Targeted Method to Identify Prokaryotic Biodegradation Genes" Platform Presentation, Battelle Remediation of Chlorinated and Recalcitrant Compounds Conference, Monterey, California, May 2006.
• De Long, S.K., M.J. Kirisits, K.A. Kinney. "Applying Molecular Tools to Water Treatment Processes: An Efficient Method to Obtain Key Gene Sequences" Platform Presentation, Water Environment Association of Texas Conference, Austin, Texas, April 2006.

• Rossmassler, K., De Long, S.K. Meta-omic-enabled biomarker identification: Biodegradation of o-xylene by a methanogenic consortium. 3rd Annual Front Range Computational & Systems Biology Symposium: Microbiome. Colorado State University, Fort Collins, CO, June, 2017.
• Wolfe, L., Larson, V., Broeckling, C.D., Prenni, J., De Long, S.K. Measuring emerging contaminant degradation in anaerobic digestion or composting systems by liquid-liquid extraction and LC-QQQ-MS. 65th American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics. Indianapolis, IN, June, 2017.
• Rossmassler, K., De Long, S.K. Meta-omic-enabled biomarker identification: biodegradation of o-xylene by a methanogenic consortium. ASM Microbe, New Orleans, LA, June, 2017.
• Galloway, S., Kim, S., Broeckling, C.D., Prenni, J., De Long, S.K. Gas chromatography-mass spectrometry method optimization for quantifying pharmaceuticals and personal care products in wastewater. Poster, Colorado State University Celebrate Undergraduate Research and Creativity- CURC, 2015.
• Bezold, Z.E., Irianni Renno, M., Blotevogel, J., Sale, T., De Long, S.K. Evidence for key role of Pseudomonas stutzeri in biodegradation of nitro- and chloroaromatic compounds in anoxic zones. Poster, American Society of Microbiology General Meeting, New Orleans, LA, May 2015.
• Irianni Renno, M., Byrne, A., Akhbari, D., Sale, T.S., De Long, S.K. Characterization of Microbial Communities Mediating Anaerobic Biodegradation of Petroleum Hydrocarbons along a Depth Transect in LNAPL Zones. Poster, American Society of Microbiology General Meeting, Denver, CO, May 2013.
• Wiles, K.M., Sharvelle, S.E., De Long, S.K. Investigation of Pathogen Disinfection and Regrowth in a Graywater Reuse Treatment System for Toilet Flushing. Poster, American Society of Microbiology General Meeting, Denver, CO, May 2013.
• Wiles, K.M., Sharvelle, S.E., De Long, S.K. Development of a Low Cost Graywater Treatment System: Disinfection and Regrowth at the Demonstration Scale. 10th Annual Rocky Mountain Section of the American Water Works Association, Student Research Conference, Golden, CO, May 2013. BEST ORAL PRESENTATION AWARD.
• Wilson, L.P., Loetscher, L.H., Sharvelle, S.E., De Long, S.K. Microbial community acclimation enhances waste hydrolysis rates under elevated ammonia and salinity conditions. Poster, American Society of Microbiology General Meeting, Denver, CO, May 2013.
• Irianni Renno, M., Akhbari, D., Byrne, A., Sale, T.S., De Long, S.K. Characterization of Microbial Communities Mediating Anaerobic Biodegradation of Petroleum Hydrocarbons along a Depth Transect in NAPL Zones. Hydrology Days, Fort Collins, CO, 2013.
• Vadheim, B., Griffin, L.P., De Long, S.K. Anaerobic Digestion of Crop Residues: Characterization and Impact Analysis of Hydrolysis Rates. Colorado Center for Biorefining and Biofuels. Aug 2012.
• Keely, S.P., Brinkman, N.E., Wiles, K., Hodgson, B., De Long, S.K., Sharvelle, S.E., Garland, J.L. Identification and Characterization of Graywater Microbial Communities by High Throughput Pyrosequencing. Water Reuse Symposium, Hollywood, FL, Sept 2012.
• Ledeker, B.M. and De Long, S.K. Determining the Effect of Mismatches on Quantitative PCR Accuracy and Developing Guidance for Designing Primers to Target Genes with Sequence Variations. Poster, American Society of Microbiology General Meeting, San Francisco, CA, May 2012.
• Lefevre, E., Pereyra, L.P., De Long, S.K., Pruden, A., and Reardon, K.F., "Response of a sulfate-reducing bioreactor microbial community to an aerobic stress event: A functional gene-based approach." Microbial Communities as Drivers of Ecosystem Complexity, Keystone Symposia, Breckenridge, CO, March, 2011.
• Zeman, N.R., De Long, S.K., Sale, T.C. "Thermally Enhanced Bioremediation of Hydrocarbon Impacted Subsurface". Hydrology Days, Colorado State University, Fort Collins, CO, 2011.
• De Long, S.K., B.A. Afshar, A.E. O'Neil, K.A. Kinney, and M.J. Kirisits. "Gene Targets for Bioreactors Treating Perchlorate-Contaminated Water" Poster Presentation, International Water Association Young Water Professionals Conference, Berkeley, California, July 2008.
• Kirisits, M. J., S. K. De Long, K. A. Kinney, S.A. Desai, and J. C. Brown. (2008) "Characterizing the Microbial Community for a Bioreactor Treating Perchlorate-Contaminated Groundwater" Proceedings of the Inorganic Contaminants Workshop. Albuquerque, New Mexico.
• De Long, S.K., K.A. Kinney, M.J. Kirisits. "Optimization of Suppression PCR for Suppressive Subtractive Hybridization (SSH) PCR cDNA Subtraction" Poster, American Society of Microbiology General Meeting, Toronto, Ontario, Canada, May 2007.
• De Long, S.K., M.J. Kirisits, K.A. Kinney. "Development of a Method to Efficiently Determine Gene Sequences Relevant to Pollutant Biodegradation" Poster, American Society of Microbiology General Meeting, New Orleans, Louisiana, May 2004.

Technical Reports:

• R1. Summers, M.D., Williams, D.W., Yazdani, R., De Long, S.K. (2015) Optimizing Biogas Production from Organic Waste through RNA/DNA Molecular Testing. Energy Innovations Small Grant Natural Gas Program (EISG) Grant #: 13-05G.
• Kirisits, M., Kinney, K., Bae, S. and De Long, S.K. (2013) Prokaryotic cDNA Subtraction: A Method to Rapidly Identify Functional Gene Biomarkers. SERDP Project ER-1563.

Invited Talks:

• De Long, S.K. "Molecular Biology Tools for Identification and Quantification of Perchlorate-Reduction Genes in Biotreatment Applications" Virginia Tech University, Blacksburg, Virginia, Oct. 22, 2010.
• De Long, S.K. "Molecular Biology Tools for Environmental Applications: Biological Perchlorate Treatment" NIST, Boulder, Colorado, April 2, 2010.
• De Long, S.K. "Development of Molecular Biology Tools for Biological Perchlorate Treatment" Stanford University, Palo Alto, California, May 23, 2008.

Thesis and Dissertation:

• De Long, S.K. (2009) "Molecular Biology Tools for Identification and Quantification of Perchlorate-Reduction Genes in Biotreatment Applications" Doctoral Dissertation, Department of Civil, Architectural, and Environmental Engineering, University of Texas at Austin.
• De Long, S.K. (2005) "Development of a Method to Efficiently Determine Prokaryotic Gene Sequences Relevant to Pollutant Biodegradation" Master’s Thesis, Department of Civil, Architectural, and Environmental Engineering, University of Texas at Austin.