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Dynamic Superresolution Imaging in Living Cells

The cortical actin cytoskeleton is a complex, highly mobile network that supports the critical cell function of trafficking, immobilizing, and localizing membrane proteins. Conventional diffraction-limited microscopy cannot resolve the characteristic distances between actin bundles. In our group, we use photoactivated localization microscopy (PALM) to image actin filaments with superresolution.

We are investigating the dynamics of the cortical actin and its role in the segregation of membrane proteins into specific microdomains. To date, we have successfully performed simultaneous actin superresolution imaging and single particle tracking of plasma membrane proteins in live human embrionic kidney (HEK) and ND7/23 cells.

 

 

 

Group members involved

Jenny Higgins

Aubrey Weigel

 

Collaborators

Mike Tamkun, Liz Akin, Jim Bamburg, Laurie Minamide, Barbara Bernstein