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Graduate Exam Abstract


David Winters

Ph.D. Final

June 26, 2014, 1:00 PM

Pheonix Design Studio (Scott Engr. 357)

Advances in single-pixel imaging toward biological applications


Abstract: In this work, we discuss two new methods for single- pixel imaging. First, we leverage advances in laser metrology and frequency synthesis to measure small shifts in the center frequency of an optical pulse. Pulses acquire such shifts when probing a transient optical susceptibility, as in impulse stimulated Raman scattering, which we use to demonstrate the technique. We analyze the limits of this technique with regard to fundamental noise, and predict detection sensitivity in these limiting cases. We then present work on imaging in two dimensions, both x—y and x—z, using single element detectors. We accomplish this by multiplexing spatial frequency projections in time, allowing rapid two dimensional imaging without an imaging detector. As we eliminate the imaging detector, the sensitivity to scattering is dramatically decreased, allowing the method to be used deep in scattering tissue. Results are shown for several geometries and experimental configurations, demonstrating imaging capabilities across a variety of sample types, including fluorescent and biological samples.

Adviser: Randy Bartels
Co-Adviser: N/A
Non-ECE Member: Elliot Bernstein, Chemistry
Member 3: Mario Marconi, Electrical & Computer Engineering
Addional Members: Ashok Prasad, Chemical & Biological Engineering

Publications:
D. R. Smith, D. G. Winters, and R. A. Bartels, "Submillisecond second harmonic holographic imaging of biological specimens in three dimensions," Proc. Natl. Acad. Sci. U. S. A., vol. 110, no. 46, pp. 18391-6, Nov. 2013. D. J. Higley, D. G. Winters, and R. A. Bartels, "Two-dimensional spatial-frequency-modulated imaging through parallel acquisition of line images," Opt. Lett., vol. 38, no. 11, pp. 1763-5, Jun. 2013. D. G. Winters, J. Speirs, E. Block, R. A. Bartels, and J. A. Squier, "High-Speed Two-Dimensional Multiphoton Microscope using Spatial Modulation," in CLEO Technical Digest, 2012, pp. 3-4. D. G. Winters, D. R. Smith, P. Schlup, and R. A. Bartels, "Measurement of orientation and susceptibility ratios using a generation holographic microscope," Biomed. Opt. Express, vol. 3, no. 9, pp. 2004-2011, 2012. D. G. Winters, R. A. Bartels, J. C. Speirs, E. Block, and J. A. Squier, "Multiphoton Microscope Using Spatially-Modulated Line-Cursor," in IEEE Photonics Conference, 2012, vol. 2, pp. 568-569. D. Smith, D. G. Winters, and R. Bartels, "High-Speed Second Harmonic Generation Holographic Imaging of Biological Specimens at over 1000 Volumes per Second," in IEEE Photonics Conference, 2012, vol. 2, pp. 2-3. D. Smith, D. G. Winters, and R. Bartels, "Label-free second harmonic generation holographic imaging of biological specimens at speeds up to 1000 volumes per second," in Conference on Lasers and Electro-Optics 2012, 2012, p. CTu3J.6. D. R. Smith, D. G. Winters, P. Schlup, and R. A. Bartels, "Hilbert reconstruction of phase-shifted second-harmonic holographic images.," Opt. Lett., vol. 37, no. 11, pp. 2052-4, Jun. 2012. E. E. Hoover, J. J. Field, D. G. Winters, M. D. Young, E. V Chandler, J. C. Speirs, J. T. Lapenna, S. M. Kim, S.-Y. Ding, R. a Bartels, J. W. Wang, and J. a Squier, "Eliminating the scattering ambiguity in multifocal, multimodal, multiphoton imaging systems.," J. Biophotonics, vol. 12, pp. 1-12, Mar. 2012. D. J. Higley, D. G. Winters, G. L. Futia, and R. A. Bartels, "Theory of diffraction effects in spatial frequency-modulated imaging.," J. Opt. Soc. Am. A, vol. 29, no. 12, pp. 2579-90, Dec. 2012. D. J. Higley, D. G. Winters, and R. A. Bartels, "Theory of diffraction and defocus effects in spatial frequency-modulated imaging," in IEEE Photonics Conference, 2012, vol. 29, no. 12, pp. 8-9. R. A. Bartels, D. R. Smith, and D. G. Winters, "Advancing second harmonic generation holography for high speed 3d sub-cellular imaging to >1000 volumes per second," in Focus on Microscopy, 2012, p. 240. D. G. Winters, P. Schlup, and R. A. Bartels, "Generation and Stability Characterization of Fiber-Based Difference Frequency Generation Tuned Through Controlled Soliton Self-Frequency-Shifting," in Advanced Solid State Photonics, 2011, p. ATuA6. G. Futia, D. G. Winters, P. Schlup, and R. A. Bartels, "Spatial frequency modulation imaging of absorption and fluorescent objects using a single element detector," in Society of Photo-Optical Instrumentation Engineers (SPIE) Conference Series, 2011, p. 79040E. G. Futia, P. Schlup, D. G. Winters, and R. A. Bartels, "Spatially-chirped modulation imaging of absorbtion and fluorescent objects on single-element optical detector," Opt. Express, vol. 19, no. 2, pp. 489-503, Jan. 2011. R. Bartels, D. Winters, D. Kupka, W. Dang, and A. Pezeshki, "Extracting Information from Optical Fields through Spatial and Temporal Modulation," in Frontiers in Optics 2011/Laser Science XXVII, 2011, p. FTuZ1. D. G. Winters, P. Schlup, and R. A. Bartels, "Subpicosecond fiber-based soliton-tuned mid-infrared source in the 9.7-14.9 μm wavelength region," Opt. Lett., vol. 35, no. 13, p. 2179, 2010. D. G. Winters, P. Schlup, and R. A. Bartels, "Soliton-tuned difference-frequency-based mid-infrared source," in 23rd Annual Meeting of the IEEE Photonics Society, 2010, pp. 175-176. R. A. Bartels, D. Winters, G. Futia, and P. Schlup, "Fiber-based , soliton-tuned femtosecond optical source mid infrared spectral region," in Conference on Lasers and Electro-Optics, 2010, pp. 1-2. D. G. Winters, P. Schlup, and R. A. Bartels, "Soliton-tuned fiber-based femtosecond optical comb source in the 10.5-15 μm wavelength region," in UFO-HFSW, 2009. D. G. Winters, P. Schlup, and R. A. Bartels, "Tunable Mid-Infrared Source based on Difference-Frequency Mixing of Soliton-Shifted Pulses," in Fall 2009 Meeting of the Four Corners Section of the American Physical Society, 2009, p. K3. D. G. Winters, P. Schlup, and R. A. Bartels, "Multi-octave spanning Fourier-transform spectrometer for ultra-broad bandwidth spectral measurements," in UFO HFSW, 2007, no. 2, pp. 1-2. D. G. Winters, P. Schlup, and R. A. Bartels, "Highly achromatic Fourier-transform spectrometer," Opt. Express, vol. 15, no. 3, pp. 1361-1368, Feb. 2007.


Program of Study:
ECE641
ECE514
PH572
GSTR600
ECE799
N/A
N/A
N/A