Graduate Exam Abstract

Ishan Thakkar

M.S. Final

March 26, 2013, 3:15 pm

Military Science 200

A plastic microfluidic device for enzymatic biosensors

Abstract: A novel microfluidic device is fabricated for application to field-portable, low scatter and economical chemical sensors and biosensors with excellent detection limits. To evaluate its utility as a chemical sensor, two pH-sensors based on two different methods of depositing fluorescenamine are demonstrated. As a proof-of- concept of the device¬ís application as a biosensor, an enzymatic Hydrogen Peroxide (H2O2)sensor is demonstrated using O2-sensitive Ru(dpp) dye as a transducer and catalase enzyme as a bio-analytical component. The H2O2 sensor exhibits a phosphorescence to scattered excitation light ratio of 100. Also, the H2O2 sensor has a detection limit of 1.2 µM, which is comparable to the best reported results of H2O2 sensors in literature.

Adviser: Dr Kevin Lear
Co-Adviser: N/A
Non-ECE Member: Dr Kenneth Reardon, Department of Chemical and Biological Engineering
Member 3: Dr George Collins, ECE
Addional Members: N/A


Program of Study:
ECE 441
ECE 471
ECE 536
ECE 548
ECE 569
ECE 573
ECE 699
STAT 511